منابع مشابه
Time-multiplexed multifocal multiphoton microscope.
We resolve the classical conflict between parallelization and axial resolution in three-dimensional fluorescence microscopy through time-multiplexed multifocal multiphoton excitation. A rotating array of microlenses on a disk splits ultrafast laser pulses in such a way that an array of high-aperture foci are created in the sample. Two rigidly mounted corotating glass disks with suitable arrays ...
متن کاملFibre-coupled multiphoton microscope with adaptive motion compensation.
To address the challenge of sample motion during in vivo imaging, we present a fibre-coupled multiphoton microscope with active axial motion compensation. The position of the sample surface is measured using optical coherence tomography and fed back to a piezo actuator that adjusts the axial location of the objective to compensate for sample motion. We characterise the system's performance and ...
متن کاملPerformance evaluation of a sensorless adaptive optics multiphoton microscope.
A wavefront sensorless adaptive optics technique was combined with a custom-made multiphoton microscope to correct for specimen-induced aberrations. A liquid-crystal-on-silicon (LCoS) modulator was used to systematically generate Zernike modes during image recording. The performance of the instrument was evaluated in samples providing different nonlinear signals and the benefit of correcting hi...
متن کاملA multiphoton microscope platform for imaging the mouse eye
PURPOSE To demonstrate the ability of multiphoton microscopy to obtain full three-dimensional high-resolution images of the intact mouse eye anterior chamber without need for enucleation. METHODS A custom multiphoton microscope was constructed and optimized for deep tissue imaging. Simultaneous two-photon autofluorescence (2PAF) and second harmonic generation (SHG) imaging were performed. A m...
متن کاملAll-Optical Scanning Acoustic Microscope
In this thesis a new instrument, the all-optical scanning acoustic microscope (O-SAM) is presented, it is a non contact scanning acoustic microscope (SAM) which uses lasers to both generate and detect surface acoustics waves (SAWs) The non contact nature of the O-SAM overcomes some difficulties associated with conventional SAMs because of the couplant and surface contact involved. This O-SAM al...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2017
ISSN: 1094-4087
DOI: 10.1364/oe.25.023399